Journal: Neural Regeneration Research

Article Title: Emerging role of microglia in the developing dopaminergic system: Perturbation by early life stress

doi: 10.4103/NRR.NRR-D-24-00742

Figure Lengend Snippet: Effects of ELS on microglia and the DA systems in key brain regions and mediated behavioral effects

Article Snippet: , IL-1 receptor antagonist , Sprague–Dawley rat, M , Intracerebral injection of LPS (1 mg/kg) at PND5 , Intracerebral injection of IL-1 receptor antagonist (0.1 mg/kg) at PND5 , SN , IL-1R antagonist reduces microglial activation and IL-1β, IL-6, TNF-α levels, attenuating dopamine neuron damage. , Pang et al., 2015.

Techniques: Inhibition

Journal: Nucleic Acids Research

Article Title: Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathways

doi: 10.1093/nar/gkaf1326

Figure Lengend Snippet: hFen1 immunodepletion increased SP-BER product. ( A ) The BER patch size for a U:A and rAP:A plasmid substrate generated by asynchronous HEK 293 FT WCE or WCE supplemented with Polβ (50 nM) and aphidicolin (100 μg/ml). Aph, aphidicolin. **** indicates P < .0001. n = 3 independent experiments and the data are means ± SD. Async, asynchronous. ( B ) The hFen1 abundance in asynchronous HEK 293 FT WCE and anti-hFen1 antibody immunodepleted WCE were determined using western blot. ID: Immunodepletion. ( C ) The BER patch size for a U:A plasmid substrate generated by asynchronous HEK 293 FT WCE, hFen1 immunodepleted WCE, hFen1 (150 nM) supplemented hFen1 immunodepleted WCE, and xFen1 (150 nM) supplemented hFen1 immunodepleted WCE. **** indicates P < .0001. ns, not significant. n = 3 independent experiments and the data are means ± SD.

Article Snippet: Rabbit polyclonal anti-hFen1 antibody (1:500, Proteintech, 14768-1-AP), rabbit polyclonal anti-xFen1 antibody (1:1000, see the “Acknowledgments” section), rabbit polyclonal anti-hPolβ antibody (1:250, Novus, NBP2-38600), rabbit polyclonal anti-GAPDH antibody (1:1000, Rockland, 600-401-A33), and IRDye 800CW labeled goat anti-rabbit IgG (1:10 000, LI-COR, 926-32211) were diluted in 1% non-fat milk in TBS-T.

Techniques: Immunodepletion, Plasmid Preparation, Generated, Western Blot